diff --git a/src/crewai/telemetry/telemetry.py b/src/crewai/telemetry/telemetry.py
index ffd78d28e..44b0c3a0d 100644
--- a/src/crewai/telemetry/telemetry.py
+++ b/src/crewai/telemetry/telemetry.py
@@ -76,10 +76,14 @@ class Telemetry:
         return cls._instance
 
     def __init__(self) -> None:
+        if hasattr(self, '_initialized'):
+            return
+        
         self.ready: bool = False
         self.trace_set: bool = False
 
         if self._is_telemetry_disabled():
+            self._initialized: bool = True
             return
 
         try:
@@ -105,6 +109,8 @@ class Telemetry:
             ):
                 raise  # Re-raise the exception to not interfere with system signals
             self.ready = False
+        finally:
+            self._initialized = True
 
     def _is_telemetry_disabled(self) -> bool:
         """Check if telemetry should be disabled based on environment variables."""
@@ -124,7 +130,7 @@ class Telemetry:
                 self.trace_set = False
 
     def _safe_telemetry_operation(self, operation):
-        if not self.ready:
+        if not self.ready or self._is_telemetry_disabled():
             return
         try:
             operation()
diff --git a/tests/telemetry/test_telemetry.py b/tests/telemetry/test_telemetry.py
index 51c5a79f1..b4943912e 100644
--- a/tests/telemetry/test_telemetry.py
+++ b/tests/telemetry/test_telemetry.py
@@ -22,6 +22,7 @@ from opentelemetry import trace
 )
 def test_telemetry_environment_variables(env_var, value, expected_ready):
     """Test telemetry state with different environment variable configurations."""
+    Telemetry._instance = None
     with patch.dict(os.environ, {env_var: value}):
         with patch("crewai.telemetry.telemetry.TracerProvider"):
             telemetry = Telemetry()
@@ -30,6 +31,7 @@ def test_telemetry_environment_variables(env_var, value, expected_ready):
 
 def test_telemetry_enabled_by_default():
     """Test that telemetry is enabled by default."""
+    Telemetry._instance = None
     with patch.dict(os.environ, {}, clear=True):
         with patch("crewai.telemetry.telemetry.TracerProvider"):
             telemetry = Telemetry()
diff --git a/tests/telemetry/test_telemetry_disable.py b/tests/telemetry/test_telemetry_disable.py
index 16c02acaa..8d42e256e 100644
--- a/tests/telemetry/test_telemetry_disable.py
+++ b/tests/telemetry/test_telemetry_disable.py
@@ -1,5 +1,5 @@
 import os
-from unittest.mock import patch
+from unittest.mock import patch, MagicMock
 
 import pytest
 
@@ -16,6 +16,7 @@ from crewai.telemetry import Telemetry
 ])
 def test_telemetry_environment_variables(env_var, value, expected_ready):
     """Test telemetry state with different environment variable configurations."""
+    Telemetry._instance = None
     with patch.dict(os.environ, {env_var: value}):
         with patch("crewai.telemetry.telemetry.TracerProvider"):
             telemetry = Telemetry()
@@ -24,7 +25,70 @@ def test_telemetry_environment_variables(env_var, value, expected_ready):
 
 def test_telemetry_enabled_by_default():
     """Test that telemetry is enabled by default."""
+    Telemetry._instance = None
     with patch.dict(os.environ, {}, clear=True):
         with patch("crewai.telemetry.telemetry.TracerProvider"):
             telemetry = Telemetry()
             assert telemetry.ready is True
+
+
+def test_telemetry_disable_after_singleton_creation():
+    """Test that telemetry operations are disabled when env var is set after singleton creation."""
+    Telemetry._instance = None
+    
+    with patch.dict(os.environ, {}, clear=True):
+        with patch("crewai.telemetry.telemetry.TracerProvider"):
+            telemetry = Telemetry()
+            assert telemetry.ready is True
+            
+            mock_operation = MagicMock()
+            telemetry._safe_telemetry_operation(mock_operation)
+            mock_operation.assert_called_once()
+            
+            mock_operation.reset_mock()
+            
+            os.environ['CREWAI_DISABLE_TELEMETRY'] = 'true'
+            
+            telemetry._safe_telemetry_operation(mock_operation)
+            mock_operation.assert_not_called()
+
+
+def test_telemetry_disable_with_multiple_instances():
+    """Test that multiple telemetry instances respect dynamically changed env vars."""
+    Telemetry._instance = None
+    
+    with patch.dict(os.environ, {}, clear=True):
+        with patch("crewai.telemetry.telemetry.TracerProvider"):
+            telemetry1 = Telemetry()
+            assert telemetry1.ready is True
+            
+            os.environ['CREWAI_DISABLE_TELEMETRY'] = 'true'
+            
+            telemetry2 = Telemetry()
+            assert telemetry2 is telemetry1
+            assert telemetry2.ready is True
+            
+            mock_operation = MagicMock()
+            telemetry2._safe_telemetry_operation(mock_operation)
+            mock_operation.assert_not_called()
+
+
+def test_telemetry_otel_sdk_disabled_after_creation():
+    """Test that OTEL_SDK_DISABLED also works when set after singleton creation."""
+    Telemetry._instance = None
+    
+    with patch.dict(os.environ, {}, clear=True):
+        with patch("crewai.telemetry.telemetry.TracerProvider"):
+            telemetry = Telemetry()
+            assert telemetry.ready is True
+            
+            mock_operation = MagicMock()
+            telemetry._safe_telemetry_operation(mock_operation)
+            mock_operation.assert_called_once()
+            
+            mock_operation.reset_mock()
+            
+            os.environ['OTEL_SDK_DISABLED'] = 'true'
+            
+            telemetry._safe_telemetry_operation(mock_operation)
+            mock_operation.assert_not_called()